Stable isotope labeling methods for protein NMR spectroscopy
نویسندگان
چکیده
0079-6 doi:10. * Co Scienc Tokyo E-m 2.1. H labeling in early studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210 2.2. Uniform N labeling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210 2.3. Amino acid type-selective Cand N-double labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 2.4. Uniform Cand N-double labeling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 2.5. Segmental labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 2.6. C-reverse labeling of aromatics in uniform Cand N-double labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 212 2.7. H random fractional labeling in uniform Cand N-double labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 214 2.8. Combination of full H-labeling and uniform Cand/or N-labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215 2.9. Backbone selective protonation in full H, C, N-labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 216 2.10. Amino acid type-selective protonation in full H, C, N-labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217 2.11. Methyl group selective protonation in full H, C, and N-labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 218 2.12. Stereo-selective labeling of prochiral methyl groups in Val and Leu residues . . . . . . . . . . . . . . . . . . . . . . . . . . . . 218 2.13. Stereospecific H labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219 2.14. SAIL (stereo-array isotope labeling) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 220 3. Sample preparation methods. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223 3.1. E. coli and other living cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223 3.2. Cell-free protein synthesis system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 224 4. Concluding remarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 224 Acknowledgements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 224 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 224
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